Composite

Part:BBa_J36838

Designed by: Perry Tsai   Group: iGEM06_Harvard   (2006-10-29)

Lipoprotein signal peptide + Outer membrane protein A, aa 46-66

This part is an assembly of the lipoprotein 20aa signal peptide and its 1st nine amino acids, with amino acids 46-66 of outer membrane protein A. When fused with a protein downstream, this surface display vehicle should express a protein on the outer surface of E. coli.

NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None